Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Hematopoietic Fas−/−→Ldlr−/− chimeric mice do not show changes in atherosclerotic lesion area, cellularity, or apoptosis. WT→Ldlr−/− (n = 15) and Fas−/−→Ldlr−/− mice (n = 11) were generated and fed with an 0.5% cholesterol diet for 16 weeks. A: Flow cytometry was performed on peripheral blood of the bone-marrow chimeric mice at the time of sacrifice. Representative profiles are shown. Green indicates background staining with no antibody in Fas−/−→Ldlr−/− mice; red indicates positive staining with CD95 antibody in WT→Ldlr−/− chimeric mice; and blue indicates lack of staining with CD95 (Fas) antibody in Fas−/−→Ldlr−/− chimeric mice. B: Atherosclerotic lesion area was determined from H&E-stained brachiocephalic artery sections (6 per mouse, 50-μm intervals) using ImageJ software. DAPI-positive cells (C) and TUNEL-positive cells (D) were counted from brachiocephalic artery sections (6 to 10 sections per mouse), using fluorescence microscopy. Each symbol represents an individual mouse; horizontal lines indicate the mean value. P > 0.05, Mann-Whitney U-test (B, C, and D).

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Flow Cytometry, Staining, Software, TUNEL Assay, Fluorescence, Microscopy, MANN-WHITNEY

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Hypercholesterolemia is increased in hematopoietic Fas−/−→Ldlr−/− chimeric mice. WT→Ldlr−/− (n = 15) and Fas−/−→Ldlr−/− mice (n = 11) were generated and fed with an 0.5% cholesterol diet for 16 weeks. A: Total plasma cholesterol levels. *P < 0.001, Student's t-test. Data are reported as means ± SD. B: Fast protein liquid chromatography profiles were performed on pooled plasma samples from WT→Ldlr−/− and Fas−/−→Ldlr−/− mice.

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Clinical Proteomics, Fast Protein Liquid Chromatography

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Microvascular inflammation is observed in hematopoietic Fas−/−→Ldlr−/− chimeric mice. Liver and lung tissue from Fas−/− and WT hematopoietic chimeras generated on Ldlr−/− background were evaluated after 16 weeks on an 0.5% cholesterol diet. Adjacent sections were stained with H&E, the macrophage antibody Mac-2, the B lymphocyte antibody B220, or the T lymphocyte antibody CD3. Original micrographs, ×20.

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Generated, Staining

Journal: The American Journal of Pathology

Article Title: Hematopoietic Fas Deficiency Does Not Affect Experimental Atherosclerotic Lesion Formation despite Inducing a Proatherogenic State

doi: 10.1016/j.ajpath.2011.02.011

Figure Lengend Snippet: Comparison of Global and Hematopoietic Fas-Deficient and FasL-Deficient Models and Their Effect in Mouse Models of Atherosclerosis

Article Snippet: Plasma cholesterol levels were measured at the Northwest Lipid Research Laboratories (Seattle, WA).

Techniques: Comparison, Western Blot, Control

Journal: Pharmaceuticals

Article Title: Nifedipine Improves the Ketogenic Diet Effect on Insulin-Resistance-Induced Cognitive Dysfunction in Rats

doi: 10.3390/ph17081054

Figure Lengend Snippet: Effect of the ND and KD with or without nifedipine (5.2 mg/kg/day, p.o.) on serum lipid profile: ( a ) Cholesterol, ( b ) triglycerides, ( c ) high-density lipoprotein (HDL), and ( d ) low-density lipoprotein (LDL) ( d ) in (IR) rats. Results are represented as median and interquartile range (min. to max. with all points) ( n = 6) and analyzed using one-way ANOVA followed by Tukey–Kramer test, with * p ≤ 0.05 vs. normal rats; # p ≤ 0.05 vs. IR + ND; and @ p ≤ 0.05 vs. IR + ketogenic diet.

Article Snippet: Following a colorimetric method, cholesterol, HDL and LDL, and TGs were measured in serum using the following kits: Cat. No. Z5030055; BioChain, Hayward, CA, USA, Cat. No. Z5030057; BioChain, Hayward, CA, USA, Cat. No. 5603-01; XpressBio, Frederick, MD, USA, respectively.

Techniques:

Journal: BMC Sports Science, Medicine and Rehabilitation

Article Title: Effects of resistance training and turmeric supplementation on reactive species marker stress in diabetic rats

doi: 10.1186/s13102-020-00194-9

Figure Lengend Snippet: Analysis of body weight, fasting plasma glucose, aspartate aminotransferase, and alanine aminotransferase of the diabetic animals group (DC) (white bar); trained diabetics (TD) group submitted to resistance training for four weeks (light gray bar); supplemented diabetics (SD) group receiving 200 mg/kg body three times per week for four weeks (dark gray bar); and trained and supplemented diabetics (TSD) group submitted to Curcuma longa supplementation and the resistance training program simultaneously (black bar). Body weight ( a ), fasting plasma glucose ( b ), aspartate aminotransferase (AST) ( c ) and alanine aminotransferase (ALT) ( d ). Data represent the mean ± standard deviation of the mean. One-way analysis of variance (ANOVA one way) and post hoc de Bonferroni tests were used. Letters on the bars represent the significant difference by one-way ANOVA followed by Bonferroni’s test among the groups as follows: body weight ( ** p ≤ 0.01 for DC vs. SD; *** p ≤ 0.001 for DC vs. TSD), glucose ( # p ≤ 0.05 for DC vs. SD; ### p ≤ 0.001 for DC vs. TSD), AST ( p ≤ 0.05 for DC vs. TSD), and ALT ( ● p ≤ 0.05 for DC vs. SD; ●●● p ≤ 0.001 for DC vs. TSD). For the DC vs. TD and SD vs. TSD groups, Student’s t-test was used. ns = no significant difference. n = 7–8 in all experimental groups

Article Snippet: The serum was used to measure the concentrations of triglycerides, total cholesterol and HDL cholesterol (HDL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), and uric acid according to the manufacturer’s procedures (Labtest®, Lagoa Santa, Minas Gerais, Brazil).

Techniques: Clinical Proteomics, Standard Deviation